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验证数据展示

  • IF Staining of HeLa using CL488-12797

    IF Staining of HeLa using CL488-12797

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 HSPA2 antibody (CL488-12797) at dilution of 1:200, CL594-Phalloidin (red).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated HSPA2 Polyclonal antibody

HSPA2 Polyclonal Antibody for IF/ICC
Cat No. CL488-12797

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC

别名

Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HSPA2 Ready-To-Use IHC Kit. HSPA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 12797-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-12797 targets HSPA2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 HSPA2 fusion protein Ag3539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 heat shock 70kDa protein 2
别名 Heat shock 70 kDa protein 2, heat shock 70kDa protein 2, HSP70 2, HSP70 3, HSPA2
计算分子量 639 aa, 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC036107
基因名称 HSPA2
Gene ID (NCBI) 3306
RRIDAB_3672550
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP54652
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HSPA2 antibody CL488-12797Download protocol
FC protocol for CL Plus 488 HSPA2 antibody CL488-12797Download protocol
Standard Protocols
Click here to view our Standard Protocols