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HSPA2 Recombinant antibody

HSPA2 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240421A2

Cat no : 83466-1-RR

Print datasheet

Synonyms

HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2

验证数据展示

  • WB analysis using 83466-1-RR

    WB analysis using 83466-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83466-1-RR (HSPA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 83466-1-RR

    WB analysis of HeLa using 83466-1-RR

    WB result of HSPA2 antibody (83466-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSPA2 transfected HeLa cells.

  • IHC staining of mouse testis using 83466-1-RR

    IHC staining of mouse testis using 83466-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPA2 antibody included
  • IHC staining of mouse testis using 83466-1-RR

    IHC staining of mouse testis using 83466-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 83466-1-RR (HSPA2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPA2 antibody included
  • Affinity and Kinetic Characterization of 83466-1-RR

    Affinity and Kinetic Characterization of 83466-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83466-1-RR against Human HSPA2 were performed. The affinity constant is 1.86 nM.

经过测试的应用

Positive WB detected inHeLa cells, A431 cells, A549 cells, HaCaT cells, NIH/3T3 cells, human kidney tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy HSPA2 Ready-To-Use IHC Kit. HSPA2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83466-1-RR targets HSPA2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen HSPA2 fusion protein Ag3539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name heat shock 70kDa protein 2
Synonyms HSP70 2, Heat shock-related 70 kDa protein 2, Heat shock protein family A member 2, Heat shock 70 kDa protein 2, 240421A2
Calculated Molecular Weight 639 aa, 70 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC036107
Gene Symbol HSPA2
Gene ID (NCBI) 3306
RRIDAB_3671096
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54652
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human HSPA2 is a member of the HSPA (HSP70) family of heat-shock proteins, encoded by the gene originally described as testis-specific. Recently, it has been reported that HSPA2 can be also expressed in human somatic tissues in a cell-type specific manner. HSPA2 is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. At physiological temperature, the HSPA2 was localized primarily in cytoplasm whereas, during heat shock, localization shifted to nucleus and nucleoli (PMID: 18452162). HSPA2 is involved in sperm maturity, function and fertility. Aberrant expression of HSPA2 in testes has been connected with male infertility. Recently, HSPA2 has attracted increased interest due to its possible involvement in carcinogenesis of non-testicular tissues. This antibody well recognized the endogenous HSPA2 protein in testis and multiple cell lines. (21373891)

实验方案

Product Specific Protocols
WB protocol for HSPA2 antibody 83466-1-RRDownload protocol
IHC protocol for HSPA2 antibody 83466-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols