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HSPA6 Monoclonal antibody

HSPA6 Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

3D12D7

Cat no : 68257-1-Ig

Print datasheet

Synonyms

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

验证数据展示

  • WB analysis using 68257-1-Ig

    WB analysis using 68257-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68257-1-Ig

    WB analysis of HeLa using 68257-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 68257-1-Ig

    WB analysis of HEK-293 using 68257-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 68257-1-Ig

    IF Staining of A431 using 68257-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using HSPA6 antibody (68257-1-Ig, Clone: 3D12D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy HSPA6 Ready-To-Use IHC Kit. HSPA6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68257-1-Ig targets HSPA6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen HSPA6 fusion protein Ag4716 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 6 (HSP70B')
Synonyms Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6
Calculated Molecular Weight 643 aa, 71 kDa
Observed Molecular Weight 68-71 kDa
GenBank Accession NumberBC035665
Gene Symbol HSPA6
Gene ID (NCBI) 3310
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP17066
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)

实验方案

Product Specific Protocols
WB protocol for HSPA6 antibody 68257-1-IgDownload protocol
IF protocol for HSPA6 antibody 68257-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Biochim Biophys Acta Mol Cell Res

PP4R1 accelerates the malignant progression of NSCLC via up-regulating HSPA6 expression and HSPA6-mediated ER stress

Authors - Xunxia Zhu
View Article