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验证数据展示

  • WB analysis using 68257-1-Ig (same clone as 68257-1-PBS)

    WB analysis using 68257-1-Ig (same clone as 68257-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 68257-1-Ig (same clone as 68257-1-PBS)

    WB analysis of HeLa using 68257-1-Ig (same clone as 68257-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 68257-1-Ig (same clone as 68257-1-PBS)

    WB analysis of HEK-293 using 68257-1-Ig (same clone as 68257-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 68257-1-Ig (HSPA6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 68257-1-Ig (same clone as 68257-1-PBS)

    IF Staining of A431 using 68257-1-Ig (same clone as 68257-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using HSPA6 antibody (68257-1-Ig, Clone: 3D12D7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68257-1-PBS in a different storage buffer formulation.

HSPA6 Monoclonal antibody, PBS Only

HSPA6 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68257-1-PBS

产品说明书

CloneNo. 3D12D7

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

WB, IF/ICC, Indirect ELISA

别名

Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HSPA6 Ready-To-Use IHC Kit. HSPA6 primary antibody included.

产品信息

68257-1-PBS targets HSPA6 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 Human
免疫原 HSPA6 fusion protein Ag4716 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 heat shock 70kDa protein 6 (HSP70B')
别名 Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B, HSP70B, HSPA6
计算分子量 643 aa, 71 kDa
观测分子量 68-71 kDa
GenBank蛋白编号BC035665
基因名称 HSPA6
Gene ID (NCBI) 3310
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP17066
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HSPA6 (also known as HSP70B) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Human HSP70B is known to be strictly inducible and shows very low or no basal expression levels in most cells. HSPA6 induction has been exploited as a reasonable biomarker of cellular stresses and its mRNA expression levels have been shown to be significantly induced against a variety of cellular stresses. This antibody was raised against the C-terminal region of human HSPA6 and it may cross-react with HSPA1 (HSP70), HSPA1L and HSPA2. (18229458)