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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

    WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

  • WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

    WB analysis using 68053-1-Ig (same clone as 68053-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68053-1-Ig (HTRA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68053-1-Ig (same clone as 68053-1-PBS)

    FC experiment of HeLa using 68053-1-Ig (same clone as 68053-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug HTRA1 Monoclonal antibody (68053-1-Ig, Clone:1B11G12) and CoraLite488-conjugated Donkey Anti-Mouse IgG(H+L)( SA00013-5)(red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68053-1-PBS in a different storage buffer formulation.

HTRA1 Monoclonal antibody, PBS Only

HTRA1 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 68053-1-PBS

产品说明书

CloneNo. 1B11G12

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, Indirect ELISA

别名

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68053-1-PBS targets HTRA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 HTRA1 fusion protein Ag31204 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 HtrA serine peptidase 1
别名 PRSS11, HtrA, EC:3.4.21.-, 1B11G12
计算分子量 51 kDa
观测分子量 51 kDa
GenBank蛋白编号NM_002775
基因名称 HTRA1
Gene ID (NCBI) 5654
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92743
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.