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验证数据展示

  • IF Staining of HepG2 using CL488-68053

    IF Staining of HepG2 using CL488-68053

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 HTRA1 antibody (CL488-68053, Clone: 1B11G12 ) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-68053

    FC experiment of HeLa using CL488-68053

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Htra1 Monoclonal Antibody (CL488-68053, Clone:1B11G12)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated HTRA1 Monoclonal antibody

HTRA1 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-68053
CloneNo. 1B11G12

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

IF/ICC, FC (Intra)

Synonyms

PRSS11, HtrA, EC:3.4.21.-, 1B11G12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68053-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68053 targets HTRA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen HTRA1 fusion protein Ag31204 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name HtrA serine peptidase 1
Synonyms PRSS11, HtrA, EC:3.4.21.-, 1B11G12
Calculated Molecular Weight 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberNM_002775
Gene Symbol HTRA1
Gene ID (NCBI) 5654
RRIDAB_3672969
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92743
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTRA1, also named as HTRA, PRSS11 and L56, belongs to the peptidase S1B family. It is a protease that regulates the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. HTRA1 represses signaling by TGF-beta family members. Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7). Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). The 50 kDa HTRA1 was found at varying levels in the majority samples tested, whereas the 42 kDa form of HTRA1 was identified in degenerated intervertebral disc protein samples(PMID:22556410). The short truncated monomer 37 kDa and dimer 74 kDa are proved (PMID:25329061). The antibody is specific to HTRA1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HTRA1 antibody CL488-68053Download protocol
FC protocol for CL Plus 488 HTRA1 antibody CL488-68053Download protocol
Standard Protocols
Click here to view our Standard Protocols