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HTRA2 Recombinant antibody, PBS Only

HTRA2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240496D1

Cat no : 83492-7-PBS

Print datasheet

Synonyms

PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108

验证数据展示

  • WB analysis using 83492-7-RR (same clone as 83492-7-PBS)

    WB analysis using 83492-7-RR (same clone as 83492-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83492-7-RR (HTRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83492-7-RR (same clone as 83492-7-PBS)

    IF Staining of HepG2 using 83492-7-RR (same clone as 83492-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-7-RR, Clone: 240496D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83492-7-RR (same clone as 83492-7-PBS)

    FC experiment of HeLa using 83492-7-RR (same clone as 83492-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-7-RR, Clone:240496D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83492-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83492-7-RR

    Affinity and Kinetic Characterization of 83492-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83492-7-RR against Human HTRA2 were performed. The affinity constant is below 1 pM.

产品信息

83492-7-PBS targets HTRA2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen HTRA2 fusion protein Ag8455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HtrA serine peptidase 2
Synonyms PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108
Calculated Molecular Weight 49 kDa
GenBank Accession NumberBC000096
Gene Symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43464
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C