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HTRA2 Recombinant antibody

HTRA2 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240496D1

Cat no : 83492-7-RR

Print datasheet

Synonyms

PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108

验证数据展示

  • WB analysis using 83492-7-RR

    WB analysis using 83492-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83492-7-RR (HTRA2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83492-7-RR

    IF Staining of HepG2 using 83492-7-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HTRA2 antibody (83492-7-RR, Clone: 240496D1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of HeLa using 83492-7-RR

    FC experiment of HeLa using 83492-7-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug HTRA2 Recombinant antibody (83492-7-RR, Clone:240496D1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83492-7-RR

    Affinity and Kinetic Characterization of 83492-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83492-7-RR against Human HTRA2 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, MCF-7 cells, Raji cells, Jurkat cells, human heart tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83492-7-RR targets HTRA2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen HTRA2 fusion protein Ag8455 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name HtrA serine peptidase 2
Synonyms PARK13, Omi stress-regulated endoprotease, OMI, HtrA serine peptidase 2, EC:3.4.21.108
Calculated Molecular Weight 49 kDa
Observed Molecular Weight36 kDa
GenBank Accession NumberBC000096
Gene Symbol HTRA2
Gene ID (NCBI) 27429
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO43464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTRA2(High temperature requirement protein A2) is also named as OMI, PRSS25 and belongs to the peptidase S1B family. HTRA2 normally exists in the mitochondria, can cause MMP3 activation in the cytosol under a cell stress condition, which can ultimately lead to demise of dopaminergic neuronal cells(PMID: 22265821). It inhibits mitochondrial superoxide generation, stabilizes mitochondrial membrane potential, and prevents apoptosis at baseline and in response to extracellular inducers of mitochondrial stress(PMID: 18772386). This protein has 4 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for HTRA2 antibody 83492-7-RRDownload protocol
IF protocol for HTRA2 antibody 83492-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols