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验证数据展示

  • WB analysis using 67168-1-Ig

    WB analysis using 67168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67168-1-Ig (HUS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 67168-1-Ig

    IHC staining of human prostate cancer using 67168-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HUS1 Monoclonal antibody

HUS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67168-1-Ig

Print datasheet

CloneNo. 1E8E9

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

Checkpoint protein HUS1, hHUS1, HUS1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, U2OS cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67168-1-Ig targets HUS1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen HUS1 fusion protein Ag28527 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name HUS1 checkpoint homolog (S. pombe)
Synonyms Checkpoint protein HUS1, hHUS1, HUS1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC007013
Gene Symbol HUS1
Gene ID (NCBI) 3364
RRIDAB_2882464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO60921
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].

实验方案

Product Specific Protocols
WB protocol for HUS1 antibody 67168-1-IgDownload protocol
IHC protocol for HUS1 antibody 67168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols