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验证数据展示

  • WB analysis using 67168-1-Ig

    WB analysis using 67168-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67168-1-Ig (HUS1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 67168-1-Ig

    IHC staining of human prostate cancer using 67168-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67168-1-Ig (HUS1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HUS1 Monoclonal antibody

HUS1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67168-1-Ig

产品说明书

CloneNo. 1E8E9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, IHC, ELISA

别名

Checkpoint protein HUS1, hHUS1, HUS1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, A549 cells, U2OS cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67168-1-Ig targets HUS1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 HUS1 fusion protein Ag28527 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 HUS1 checkpoint homolog (S. pombe)
别名 Checkpoint protein HUS1, hHUS1, HUS1
计算分子量 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC007013
基因名称 HUS1
Gene ID (NCBI) 3364
RRIDAB_2882464
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO60921
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hus1 belongs to the Rad family, form a heterotrimeric clamp with Rad1and Rad9 that acts as a putative sensor for DNA damage. This complex plays an apical role in the DNA damage response, loss of a functional complex affects many downstream checkpoint pathways [PMID: 11340080]. It is also a key complex involved in the activation of the ATR pathway. 9-1-1 forms a ring-shaped heterotrimer, resembling the homotrimeric sliding clamp, proliferating cell nuclear antigen (PCNA) [PMID: 19535328].

实验方案

Product Specific Protocols
WB protocol for HUS1 antibody 67168-1-IgDownload protocol
IHC protocol for HUS1 antibody 67168-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols