- Featured Product
- KD/KO Validated
HYAL2 Polyclonal antibody
HYAL2 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, human heart tissue, human lung tissue, mouse liver tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
产品信息
51148-1-AP targets HYAL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IF, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | HYAL2 fusion protein Ag0582 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hyaluronoglucosaminidase 2 |
| Synonyms | Hyal 2, HYAL2, Hyaluronidase 2, hyaluronoglucosaminidase 2, LuCa 2, LUCA2, Lung carcinoma protein 2 |
| Calculated Molecular Weight | 54 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC000692 |
| Gene Symbol | HYAL2 |
| Gene ID (NCBI) | 8692 |
| RRID | AB_2123391 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12891 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hyaluronic acid (HA) is a glycosaminoglycan that is believed to have numerous important biologic functions, including modulation of cell proliferation, migration, and differentiation, as well as the regulation of extracellular water and protein homeostasis. It is also an integral structural component of cartilage and other tissues and acts as a lubricant in joints. Hyaluronidases are a family of enzymes that catalyse the degradation of HA. In humans, there are five functional hyaluronidases: HYAL1, HYAL2, HYAL3, HYAL4 and HYAL5 (also known as SPAM1 or PH-20); plus a pseudogene, HYAL6 (also known as HYALP1). HYAL2 is present in many tissues except the adult brain (PMID: 11731268). HYAL2 can be exposed to cell surfaces that bind to the plasma membrane via GPI anchors (PMID: 11731268).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HYAL2 antibody 51148-1-AP | Download protocol |
| IHC protocol for HYAL2 antibody 51148-1-AP | Download protocol |
| IF protocol for HYAL2 antibody 51148-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Dev Cell High-Molecular-Weight Hyaluronan Is a Hippo Pathway Ligand Directing Cell Density-Dependent Growth Inhibition via PAR1b.
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