HYAL4 Polyclonal antibody
HYAL4 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, NIH/3T3 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
18139-1-AP targets HYAL4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | HYAL4 fusion protein Ag12766 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | hyaluronoglucosaminidase 4 |
| Synonyms | Hyal 4, HYAL4, hyaluronoglucosaminidase 4 |
| Calculated Molecular Weight | 481 aa, 54 kDa |
| Observed Molecular Weight | 60-65 kDa |
| GenBank Accession Number | BC104788 |
| Gene Symbol | HYAL4 |
| Gene ID (NCBI) | 23553 |
| RRID | AB_2878506 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q2M3T9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hyaluronic acid (HA), a glycosaminoglycan that is ubiquitously present in the extracellular space of higher animals, maintains matrix structure and controls cellular functions such as proliferation, differentiation, and locomotion. Hyal enzymes that catabolize HA, are involved in development and tumorigenesis.HYAL4, a member of the Hyal family, is a chondroitin sulfate (CS)-specific endo-β-acetylgalactosaminidase. The expression of hHYAL4 mRNA is not ubiquitous but restricted to placenta, skeletal muscle, and testis (PMID: 23086929). This polyclonal antibody raised against 29-460aa of human HYAL4. The apparent molecular weight is larger than the calculated molecular weight of 54 kDa, which is likely due to posttranslational modification, presumably by glycosylation (PMID: 19889881).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HYAL4 antibody 18139-1-AP | Download protocol |
| IHC protocol for HYAL4 antibody 18139-1-AP | Download protocol |
| IP protocol for HYAL4 antibody 18139-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Appl Biochem Biotechnol Kunzea Ericoides (Kanuka) Leaf Extracts Show Moisturisation, Antioxidant, and UV Protection Effects in HaCaT Cells and Anti-melanogenesis Effects in B16F10 Cells |
