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验证数据展示

  • IF Staining of human appendicitis using CL594-14537

    IF Staining of human appendicitis using CL594-14537

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®594 Hemoglobin Alpha antibody (CL594-14537) at dilution of 1:200.

  • FC experiment of TF-1 using CL594-14537

    FC experiment of TF-1 using CL594-14537

    1X10^6 TF-1 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Hemoglobin Alpha (CL594-14537) (red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated Hemoglobin Alpha Polyclonal antibody

Hemoglobin Alpha Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-14537

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P, FC (Intra)

别名

HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inTF-1 cells
For other applications, we recommend the unconjugated version of this antibody, 14537-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14537 targets Hemoglobin Alpha in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Hemoglobin Alpha fusion protein Ag6040 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, alpha 1
别名 HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin
计算分子量 15 kDa
观测分子量 14-16 kDa, 27 kDa
GenBank蛋白编号BC050661
基因名称 HBA1
Gene ID (NCBI) 3039
RRIDAB_3673426
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP69905
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

实验方案

Product Specific Protocols
IF protocol for CL594 Hemoglobin Alpha antibody CL594-14537Download protocol
Standard Protocols
Click here to view our Standard Protocols