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验证数据展示

  • IF Staining of human appendicitis using CL594-14537

    IF Staining of human appendicitis using CL594-14537

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite®594 Hemoglobin Alpha antibody (CL594-14537) at dilution of 1:200.

  • FC experiment of TF-1 using CL594-14537

    FC experiment of TF-1 using CL594-14537

    1X10^6 TF-1 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Hemoglobin Alpha (CL594-14537) (red), or 0.4 ug CoraLite®594-conjugated Rabbit IgG control Rabbit PolyAb (CL594-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Hemoglobin Alpha Polyclonal antibody

Hemoglobin Alpha Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-14537

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Synonyms

HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue
Positive FC (Intra) detected inTF-1 cells
For other applications, we recommend the unconjugated version of this antibody, 14537-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-14537 targets Hemoglobin Alpha in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Hemoglobin Alpha fusion protein Ag6040 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name hemoglobin, alpha 1
Synonyms HBA1, Hemoglobin alpha chain, Hemoglobin a, HBA2, Alpha-globin
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 14-16 kDa, 27 kDa
GenBank Accession NumberBC050661
Gene Symbol HBA1
Gene ID (NCBI) 3039
RRIDAB_3673426
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP69905
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBA1 (hemoglobin alpha chain) protein is a alpha-type chain of hemoglobin encoded by two independent genes (HBA1 and HBA2) whose coding sequences are identical. Two alpha chains coupled with two beta chains constitute the adult hemoglobin (HbA or α2β2). HBA1 is also the component of fetal hemoglobin (HbF or α2γ2). This antibody detects a major band around 14-16 kDa in the western blot analysis of heart tissue and K-562 cells. A higher band around 26-27 kDa can also be observed occasionally, which may represents the dimer form of HBA1 (PMID: 20836851). This antibody may cross-react with other hemoglobin chains.

实验方案

Product Specific Protocols
IF protocol for CL594 Hemoglobin Alpha antibody CL594-14537Download protocol
Standard Protocols
Click here to view our Standard Protocols