Hep G2 nuclear extract

2 x 100 µg of Hep G2 nuclear extract at 2.5 µg/µl.

Hep G2 nuclear extract was prepared from cell cultures of the human hepatocellular liver carcinoma Hep G2 cell line. These cells are epithelial in morphology and can be induced to form polarized epithelium under appropriate culture conditions. Hep G2 cells are most commonly used for studies related to hepatocyte function. Because of their ability to differentiate into polarized epithelium, these cells are often used to investigate intracellular protein trafficking, specifically in relation to human liver diseases. Hep G2 is the most commonly used cell line for studying the regulation of hepatic protein synthesis, particularly the synthesis of acute phase proteins after the onset of a systemic inflammatory response. In addition, these cells are used to study liver metabolism, liver cancers, liver regeneration, liver cytotoxicity, apoptosis and as a model for hepatitis B virus (HBV) viral etiology.

Hep G2 nuclear extract is specifically recommended for studies related to 1) human liver diseases, cancers and drug therapies, 2) HBV etiology, 3) signaling pathways, and 4) apoptosis.

Human Liver (hepatocellular carcinoma)

Hep G2 nuclear extract is supplied in Dilution Buffer (20 mM Hepes (pH 7.9), 100 mM KCl, 1 mM MgCl₂, 20% glycerol, 0.5 mM PMSF and 0.5 mM DTT).

Extracts have been quality control tested by Western blot and TransAM^® HNF Family Kits.