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Hexokinase 2 Monoclonal antibody, PBS Only

Hexokinase 2 Monoclonal Antibody for WB,Indirect ELISA,IHC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC

Conjugate

Unconjugated

CloneNo.

2A11C3

Cat No : 66974-1-PBS

Print datasheet

Synonyms

DKFZp686M1669, hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase

验证数据展示

  • WB analysis using 66974-1-Ig (same clone as 66974-1-PBS)

    WB analysis using 66974-1-Ig (same clone as 66974-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse skeletal muscle using 66974-1-Ig (same clone as 66974-1-PBS)

    IHC staining of mouse skeletal muscle using 66974-1-Ig (same clone as 66974-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66974-1-Ig (Hexokinase 2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66974-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HK2 antibody included
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.

产品信息

66974-1-PBS targets Hexokinase 2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen Hexokinase 2 fusion protein Ag16895 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexokinase 2
Synonyms DKFZp686M1669, hexokinase 2, Hexokinase type II, HK II, HK2, HKII, HXK2, Muscle form hexokinase
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC021116
Gene Symbol HK2
Gene ID (NCBI) 3099
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52789
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.