验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, NIH/3T3 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, 4T1 cells |
| Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:375-1:1500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66974-1-Ig targets Hexokinase 2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Immunogen | Hexokinase 2 fusion protein Ag16895 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | hexokinase 2 |
| Synonyms | HK2, HK 2, Hexokinase-B, Hexokinase-2, hexokinase2 |
| Calculated Molecular Weight | 102 kDa |
| Observed Molecular Weight | 102 kDa |
| GenBank Accession Number | BC021116 |
| Gene Symbol | Hexokinase 2 |
| Gene ID (NCBI) | 3099 |
| RRID | AB_2882294 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P52789 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236). The antibody also recognizes Hexokinase 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Hexokinase 2 antibody 66974-1-Ig | Download protocol |
| IHC protocol for Hexokinase 2 antibody 66974-1-Ig | Download protocol |
| IF protocol for Hexokinase 2 antibody 66974-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
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Cell Metab High dietary fructose promotes hepatocellular carcinoma progression by enhancing O-GlcNAcylation via microbiota-derived acetate | ||
Small A Constant Filgotinib Delivery Adhesive Platform Based on Polyethylene Glycol (PEG) Hydrogel for Accelerating Wound Healing via Restoring Macrophage Mitochondrial Homeostasis | ||
Emerg Microbes Infect The regulation of cell homeostasis and antiviral innate immunity by autophagy during classical swine fever virus infection | ||
Clin Transl Med USP7 promotes non-small-cell lung cancer cell glycolysis and survival by stabilizing and activating c-Abl | ||
Br J Pharmacol HSP90β shapes the fate of Th17 cells with the help of glycolysis-controlled methylation modification | ||
Acta Pharmacol Sin Bmal1 inhibits phenotypic transformation of hepatic stellate cells in liver fibrosis via IDH1/α-KG-mediated glycolysis. |