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CoraLite®594-conjugated Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A11C3

Cat no : CL594-66974

Print datasheet

Synonyms

验证数据展示

  • WB analysis using CL594-66974

    WB analysis using CL594-66974

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66974 (Hexokinase 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL594-66974

    FC experiment of HepG2 using CL594-66974

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite®594 Hexokinase 2 Monoclonal Antibody (CL594-66974, Clone:2A11C3)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66974-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66974 targets Hexokinase 2 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen Hexokinase 2 fusion protein Ag16895 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexokinase 2
Synonyms
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC021116
Gene Symbol Hexokinase 2
Gene ID (NCBI) 3099
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52789
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

实验方案

Product Specific Protocols
WB protocol for CL594 Hexokinase 2 antibody CL594-66974Download protocol
Standard Protocols
Click here to view our Standard Protocols