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验证数据展示

  • WB analysis using CL594-66974

    WB analysis using CL594-66974

    Various lysates were subjected to SDS PAGE followed by western blot with CL594-66974 (Hexokinase 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of HepG2 using CL594-66974

    FC experiment of HepG2 using CL594-66974

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite®594 Hexokinase 2 Monoclonal Antibody (CL594-66974, Clone:2A11C3)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®594-conjugated Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for WB
Cat No. CL594-66974

产品说明书

CloneNo. 2A11C3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66974-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66974 targets Hexokinase 2 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 Hexokinase 2 fusion protein Ag16895 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 hexokinase 2
别名
计算分子量 102 kDa
观测分子量 102 kDa
GenBank蛋白编号BC021116
基因名称 Hexokinase 2
Gene ID (NCBI) 3099
RRIDAB_3673510
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP52789
储存缓冲液 PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

实验方案

Product Specific Protocols
WB protocol for CL594 Hexokinase 2 antibody CL594-66974Download protocol
Standard Protocols
Click here to view our Standard Protocols