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验证数据展示

  • FC experiment of HepG2 using CL488-66974

    FC experiment of HepG2 using CL488-66974

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Hexokinase 2 Monoclonal Antibody (CL488-66974, Clone:2A11C3)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using CL488-66974

    WB analysis using CL488-66974

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66974 (Hexokinase 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated Hexokinase 2 Monoclonal antibody

Hexokinase 2 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-66974
CloneNo. 2A11C3

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra)

Synonyms

HK2, Hexokinase-B, Hexokinase-2, hexokinase2, Hexokinase type II

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HK2 Ready-To-Use IHC Kit. HK2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66974-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66974 targets Hexokinase 2 in WB, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Hexokinase 2 fusion protein Ag16895 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name hexokinase 2
Synonyms HK2, Hexokinase-B, Hexokinase-2, hexokinase2, Hexokinase type II
Calculated Molecular Weight 102 kDa
Observed Molecular Weight 102 kDa
GenBank Accession NumberBC021116
Gene Symbol Hexokinase 2
Gene ID (NCBI) 3099
RRIDAB_3672930
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP52789
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hexokinase 2 (HK2), a rate-limiting enzyme in the first step of glycolysis pathway, expresses at high level in cancer cells compared with normal cells. HK2 provides a new target for cancer therapy due to its pivotal role in tumor tumourigenic and metastatic process. HK1 is constitutively expressed in most mammalian adult tissues. HK2, however, although is abundantly expressed in embryonic tissues, is expressed at high levels only in limited number of adult tissues such as adipose, skeletal, and cardiac muscles. (PMID: 29305912, PMID: 28427443, PMID: 23911236)

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 Hexokinase 2 antibody CL488-66974Download protocol
Standard Protocols
Click here to view our Standard Protocols