Mouse Hif1a Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230101E8

Cat No : 82989-4-PBS

Synonyms

AA959795, bHLHe78, Hif1a, HIF1alpha, MOP1

WB analysis of NIH/3T3 using 82989-4-RR (same clone as 82989-4-PBS)
Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82989-4-PBS in a different storage buffer formulation.
Affinity experiments of 82989-4-RR
Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.

Size

The immunogen of 82989-4-PBS is Hif1a Fusion Protein expressed in E. coli.

Tested Applications	WB, Indirect ELISA Application Description
Tested Reactivity	Mouse
Immunogen	Hif1a fusion protein Ag33620 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	hypoxia inducible factor 1, alpha subunit
Synonyms	AA959795, bHLHe78, Hif1a, HIF1alpha, MOP1
Calculated Molecular Weight	93 kDa
GenBank Accession Number	NM_001313919
Gene Symbol	Hif1a
Gene ID (NCBI)	15251
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	Q61221
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.