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验证数据展示

  • WB analysis of NIH/3T3 using 82989-4-RR

    WB analysis of NIH/3T3 using 82989-4-RR

    Cobalt Chloride treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 82989-4-RR (Hif1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82989-4-RR

    Affinity and Kinetic Characterization of 82989-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82989-4-RR against Mouse Hif1a were performed. The affinity constant is below 1 pM.

Hif-1 alpha Recombinant antibody

Hif-1 alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82989-4-RR

产品说明书

CloneNo. 230101E8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, ELISA

别名

Hif1a, 230101E8, Hypoxia inducible factor 1 alpha, HIF1α, HIF1alpha

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCobalt Chloride treated NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82989-4-RR targets Hif-1 alpha in WB, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse
免疫原 Hif-1 alpha fusion protein Ag33620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 hypoxia inducible factor 1, alpha subunit
别名 Hif1a, 230101E8, Hypoxia inducible factor 1 alpha, HIF1α, HIF1alpha
计算分子量93 kDa
观测分子量120 kDa
GenBank蛋白编号NM_001313919
基因名称 Hif1a
Gene ID (NCBI) 15251
RRIDAB_3670737
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ61221
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Hif-1 alpha antibody 82989-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols