Histone H2A.X Monoclonal antibody
Histone H2A.X Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
3E11B2
验证数据展示
经过测试的应用
Positive WB detected in | Saos-2 cells, MDA-MB-231 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68888-1-Ig targets Histone H2A.X in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | H2A histone family, member X |
Synonyms | H2AFX, Histone H2A, H2AX, H2A.X, H2A histone family, member X |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 17 kDa |
GenBank Accession Number | BC013416 |
Gene Symbol | Histone H2A.X |
Gene ID (NCBI) | 3014 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P16104 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for Histone H2A.X antibody 68888-1-Ig | Download protocol |
IF protocol for Histone H2A.X antibody 68888-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |