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Histone H2A.X Monoclonal antibody

Histone H2A.X Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3E11B2

Cat No : 68888-1-Ig

Print datasheet

Synonyms

H2AFX, Histone H2A, H2AX, H2A.X, H2A histone family, member X

验证数据展示

  • WB analysis using 68888-1-Ig

    WB analysis using 68888-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68888-1-Ig (Histone H2A.X antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68888-1-Ig

    IF Staining of HepG2 using 68888-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Histone H2A.X antibody (68888-1-Ig, Clone: 3E11B2 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

经过测试的应用

Positive WB detected inSaos-2 cells, MDA-MB-231 cells, HeLa cells, LNCaP cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy H2AX Ready-To-Use IHC Kit. H2AX primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68888-1-Ig targets Histone H2A.X in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name H2A histone family, member X
Synonyms H2AFX, Histone H2A, H2AX, H2A.X, H2A histone family, member X
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC013416
Gene Symbol Histone H2A.X
Gene ID (NCBI) 3014
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP16104
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H2A.X belongs to the histone H2A family, which is synthesized in G1 and S phases. It is involved in nucleosomal organization of chromatin together with other histone proteins and is especially important for recombination between immunoglobulin switch regions. H2A.X becomes phosphorylated on serine 139 (to form gamma-H2AFX or H2AX139ph) in response to DNA double strand breaks (DSBs) generated by exogenous genotoxic agents and by stalled replication forks, which promotes DNA repair and maintains genomic stability. The calculated molecular weight of H2AX is 15 kDa, but the ubiquitinated H2A.X is about 22 kDa.

实验方案

Product Specific Protocols
WB protocol for Histone H2A.X antibody 68888-1-IgDownload protocol
IF protocol for Histone H2A.X antibody 68888-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols