• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Histone H2B Monoclonal antibody, PBS Only

Histone H2B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

zebrafish, chicken, rat, mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12G3

Cat no : 68393-1-PBS

Print datasheet

Synonyms

HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B

验证数据展示

  • WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68393-1-Ig (Histone H2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    FC experiment of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H2B (68393-1-Ig, Clone:1E12G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

产品信息

68393-1-PBS targets Histone H2B in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with zebrafish, chicken, rat, mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity zebrafish, chicken, rat, mouse, human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone cluster 2, H2be
Synonyms HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16778
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).