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Histone H2B Monoclonal antibody, PBS Only

Histone H2B Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Zebrafish, Chicken

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1E12G3

Cat No : 68393-1-PBS

Print datasheet

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

验证数据展示

  • WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    WB analysis using 68393-1-Ig (same clone as 68393-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68393-1-Ig (Histone H2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    IHC staining of human lung cancer using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    IF Staining of HeLa using 68393-1-Ig (same clone as 68393-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68393-1-PBS in a different storage buffer formulation.

产品信息

68393-1-PBS targets Histone H2B in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.

Tested Applications WB, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Zebrafish, Chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone cluster 2, H2be
Synonyms GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol HIST2H2BE
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16778
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).