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Histone H2B Monoclonal antibody

Histone H2B Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, chicken, zebrafish

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E12G3

Cat no : 68393-1-Ig

Print datasheet

Synonyms

HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B

验证数据展示

  • WB analysis using 68393-1-Ig

    WB analysis using 68393-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68393-1-Ig (Histone H2B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 68393-1-Ig

    IHC staining of human lung cancer using 68393-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 68393-1-Ig

    IHC staining of human lung cancer using 68393-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68393-1-Ig (Histone H2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 68393-1-Ig

    IF Staining of HeLa using 68393-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone H2B antibody (68393-1-Ig, Clone: 1E12G3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • FC experiment of HeLa using 68393-1-Ig

    FC experiment of HeLa using 68393-1-Ig

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Histone H2B (68393-1-Ig, Clone:1E12G3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, chicken cerebellum tissue, zebrafish tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68393-1-Ig targets Histone H2B in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, chicken, zebrafish samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat, chicken, zebrafish
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone cluster 2, H2be
Synonyms HIST2H2BE, H2BC21, H2B/q, H2B.1, H2B
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16778
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

实验方案

Product Specific Protocols
WB protocol for Histone H2B antibody 68393-1-IgDownload protocol
IHC protocol for Histone H2B antibody 68393-1-IgDownload protocol
IF protocol for Histone H2B antibody 68393-1-IgDownload protocol
FC protocol for Histone H2B antibody 68393-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Epigenetics

Herbal medicine formula Huazhuo Tiaozhi granule ameliorates dyslipidaemia via regulating histone lactylation and miR-155-5p biogenesis

Authors - Xiangjun Yin
View Article