CoraLite® Plus 488-conjugated Histone H2B Monoclonal antibody
Histone H2B Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Zebrafish, Chicken
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E12G3
验证数据展示
经过测试的应用
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 68393-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-68393 targets Histone H2B in IF applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.
| Tested Applications | IF/ICC Application Description |
| Tested Reactivity | Human, Mouse, Rat, Zebrafish, Chicken |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | histone cluster 2, H2be |
| Synonyms | GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-17 kDa |
| GenBank Accession Number | BC005827 |
| Gene Symbol | HIST2H2BE |
| Gene ID (NCBI) | 8349 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q16778 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Histone H2B antibody CL488-68393 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |