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验证数据展示

  • IF Staining of HeLa using CL488-68393

    IF Staining of HeLa using CL488-68393

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H2B antibody (CL488-68393, Clone: 1E12G3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Histone H2B Monoclonal antibody

Histone H2B Monoclonal Antibody for IF/ICC
Cat No. CL488-68393

Print datasheet

CloneNo. 1E12G3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Zebrafish, Chicken

Applications

IF/ICC

Synonyms

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68393-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68393 targets Histone H2B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat, Zebrafish, Chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name histone cluster 2, H2be
Synonyms GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14-17 kDa
GenBank Accession NumberBC005827
Gene Symbol Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3084480
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16778
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Histone H2B antibody CL488-68393Download protocol
Standard Protocols
Click here to view our Standard Protocols