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验证数据展示

  • IF Staining of HeLa using CL488-68393

    IF Staining of HeLa using CL488-68393

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Histone H2B antibody (CL488-68393, Clone: 1E12G3 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated Histone H2B Monoclonal antibody

Histone H2B Monoclonal Antibody for IF/ICC
Cat No. CL488-68393

产品说明书

CloneNo. 1E12G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse, Rat, Zebrafish, Chicken

应用

IF/ICC

别名

GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 68393-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68393 targets Histone H2B in IF/ICC applications and shows reactivity with Human, Mouse, Rat, Zebrafish, Chicken samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human, Mouse, Rat, Zebrafish, Chicken
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 histone cluster 2, H2be
别名 GL105, H2B, H2B.1, H2B/q, H2BFQ, HIST2H2BE, histone cluster 2, H2be, Histone H2B, Histone H2B GL105, Histone H2B type 2 E, Histone H2B.q
计算分子量 14 kDa
观测分子量 14-17 kDa
GenBank蛋白编号BC005827
基因名称 Histone H2B
Gene ID (NCBI) 8349
RRIDAB_3084480
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16778
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are nuclear proteins that are classified into five major protein groups: histones H2A, H2B, H3, and H4 are known as the core histones. Post-translationally modified H2B proteins can modulate the nucleosome/chromatin structure or DNA accessibility to affect the transcriptional pathways linked to embryonic development and cell differentiation. Monoubiquitination of histone H2B has emerged as an important chromatin modification with roles not only in transcription but also in cell differentiation, DNA repair or mRNA processing(PMID: 25027370).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Histone H2B antibody CL488-68393Download protocol
Standard Protocols
Click here to view our Standard Protocols