• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 81719-2-RR

    WB analysis using 81719-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81719-2-RR (Hsc70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 81719-2-RR

    WB analysis of HeLa using 81719-2-RR

    WB result of Hsc70 antibody (81719-2-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Hsc70 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

Hsc70 Recombinant antibody

Hsc70 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 81719-2-RR

产品说明书

CloneNo. 3H9

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, MCF-7 cells, PC-12 cells
Planning an IHC experiment? We recommend our IHCeasy HSC70/HSPA8 Ready-To-Use IHC Kit. HSC70/HSPA8 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81719-2-RR targets Hsc70 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 Hsc70 fusion protein Ag1018 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heat shock 70kDa protein 8
别名 Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71
计算分子量 70 kDa
观测分子量 70 kDa
GenBank蛋白编号BC007276
基因名称 HSC70
Gene ID (NCBI) 3312
RRIDAB_3086458
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11142
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

实验方案

Product Specific Protocols
WB protocol for Hsc70 antibody 81719-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols