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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Hsc70 Monoclonal antibody

Hsc70 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A4B3

Cat no : CL488-66442

Print datasheet

Synonyms

Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71

验证数据展示

  • IF Staining of HEK-293 using CL488-66442

    IF Staining of HEK-293 using CL488-66442

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 488 Hsc70 antibody (CL488-66442, Clone: 1A4B3 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy HSC70/HSPA8 Ready-To-Use IHC Kit. HSC70/HSPA8 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66442-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66442 targets Hsc70 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen Hsc70 fusion protein Ag17910 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heat shock 70kDa protein 8
Synonyms Heat shock 70 kDa protein 8, heat shock 70kDa protein 8, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, HSPA8, LAP1, NIP71
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC007276
Gene Symbol HSC70
Gene ID (NCBI) 3312
RRIDAB_2919326
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP11142
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSPA8 (also known as HSC70) is a member of the HSPA (HSP70) family of heat-shock proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. HSPA8 is a constitutively expressed cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Recently it has been reported that HSPA8 can interact with α-synuclein, the critical pathological protein of Parkinson's disease, indicating its implication in neurodegenerative disease. (PMID: 21832061)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Hsc70 antibody CL488-66442Download protocol
Standard Protocols
Click here to view our Standard Protocols