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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated HuR/ELAVL1 Polyclonal antibody

HuR/ELAVL1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-11910

Print datasheet

Synonyms

ELAVL1, Hua, Hu antigen R, ELAV-like protein 1, ELAVL 1

验证数据展示

  • IF Staining of MCF-7 using CL488-11910

    IF Staining of MCF-7 using CL488-11910

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 HuR/ELAVL1 antibody (CL488-11910) at dilution of 1:200.

  • IF Staining of HeLa using CL488-11910

    IF Staining of HeLa using CL488-11910

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 HuR antibody (CL488-11910) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-11910

    FC experiment of MCF-7 using CL488-11910

    1X10^6 MCF-7 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human HuR/ELAVL1 (CL488-11910) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells, MCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 11910-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-11910 targets HuR/ELAVL1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen HuR/ELAVL1 fusion protein Ag2249 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R)
Synonyms ELAVL1, Hua, Hu antigen R, ELAV-like protein 1, ELAVL 1
Calculated Molecular Weight 326 aa, 36 kDa
Observed Molecular Weight 30-37 kDa
GenBank Accession NumberBC003376
Gene Symbol HuR/ELAVL1
Gene ID (NCBI) 1994
RRIDAB_2919042
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15717
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HuR/ELAVL1 antibody CL488-11910Download protocol
Standard Protocols
Click here to view our Standard Protocols