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Recombinant Human TNFR1/CD120a protein (His Tag)

ED50

0.8-3.2 ng/mL

Species

Human

Purity

>95 %, SDS-PAGE

GeneID

7132

Accession

P19438-1

Cat No : Eg0531

Print datasheet

Synonyms

CD120a, FPF, p55, p55-R, p60, TBP1, TNF RI, TNFAR, TNF-R, TNF-R1, TNFR55, TNF-R55, TNFR60, TNF-R-I, TNFRSF1A

验证数据展示

  • Purity of Recombinant Human TNFR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human TNFR1 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.

    Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.

Technical Specifications

Purity >95 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human TNFR1 (His tag) at 0.2 μg/mL (100 μL/well) can bind Human TNF alpha (GST tag) with a linear range of 0.8-3.2 ng/mL.
Source HEK293-derived Human TNFR1 protein Ile22-Thr211 (Accession# P19438-1) with a His tag at the C-terminus.
Predicted Molecular Mass 24 kDa
SDS-PAGE 32-48 kDa
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Tumor necrosis factor (TNF) is a multifunctional cytokine that plays a key role in regulating inflammation, immune functions, host defense, and apoptosis. TNF signals through two distinct cell surface receptors, TNFR1 (TNFRSF1A, CD120a) and TNFR2 (TNFRSF1B, CD120b). TNFR1, which contains a death domain (DD) within its intracytoplasmic region, is thought to be the key receptor for TNF signaling. This receptor can be released to the extracellular space by two mechanisms, ectodomain shedding and constitutive release of full-length 55 kDa TNFR1. Soluble TNFR1 (sTNFR1) could function as TNF-binding protein, inhibiting TNF bioactivity.

References:

1.  Islam A, et al. (2006). J Biol Chem. 281(10):6860-73.
2.  Aggarwal BB, et al. (2012). Blood. 119(3):651-65.
3.  Aderka D , et al. (2012). J Exp Med. 175(2):323-9.