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验证数据展示

  • IF Staining of SH-SY5Y using CL488-27206

    IF Staining of SH-SY5Y using CL488-27206

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CoraLite® Plus 488 Huntingtin antibody (CL488-27206) at dilution of 1:200.

CoraLite® Plus 488-conjugated Huntingtin Polyclonal antibody

Huntingtin Polyclonal Antibody for IF/ICC
Cat No. CL488-27206

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy HTT Ready-To-Use IHC Kit. HTT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 27206-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27206 targets Huntingtin in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Huntingtin fusion protein Ag25922 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name huntingtin
Synonyms HD, HD protein, HTT, huntingtin, Huntington disease protein, IT15
Calculated Molecular Weight 348 kDa
GenBank Accession NumberNM_002111
Gene Symbol Huntingtin
Gene ID (NCBI) 3064
RRIDAB_3084108
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42858
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HTT(huntingtin), also named as HD and IT15, belongs to the huntingtin family. HTT may play a role in microtubule-mediated transport or vesicle function. Defects in HTT are the cause of Huntington disease (HD) which is an autosomal dominant neurodegenerative disorder characterized by involuntary movements (chorea), general motor impairment, psychiatric disorders and dementia.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Huntingtin antibody CL488-27206Download protocol
Standard Protocols
Click here to view our Standard Protocols