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CoraLite®594-conjugated IA-2/PTPRN Monoclonal antibody

IA-2/PTPRN Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1D3F5

Cat no : CL594-66045

Print datasheet

Synonyms

IA-2, PTPRN, 1D3F5, ICA512-transmembrane fragment, ICA512-N-terminal fragment

验证数据展示

  • IF Staining of HepG2 using CL594-66045

    IF Staining of HepG2 using CL594-66045

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CL594-66045 (IA-2/PTPRN antibody) at dilution of 1:100.

  • FC experiment of HEK-293 using CL594-66045

    FC experiment of HEK-293 using CL594-66045

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human IA-2/PTPRN (CL594-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of Y79 using CL594-66045

    FC experiment of Y79 using CL594-66045

    1X10^6 Y79 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human IA-2/PTPRN (CL594-66045, Clone:1D3F5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHEK-293 cells, Y79 cells
Planning an IHC experiment? We recommend our IHCeasy PTPRN Ready-To-Use IHC Kit. PTPRN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66045-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66045 targets IA-2/PTPRN in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen IA-2/PTPRN fusion protein Ag17909 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, N
Synonyms IA-2, PTPRN, 1D3F5, ICA512-transmembrane fragment, ICA512-N-terminal fragment
Calculated Molecular Weight 105 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC007713
Gene Symbol IA-2/PTPRN
Gene ID (NCBI) 5798
RRIDAB_2883490
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16849
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

实验方案

Product Specific Protocols
IF protocol for CL594 IA-2/PTPRN antibody CL594-66045Download protocol
FC protocol for CL594 IA-2/PTPRN antibody CL594-66045Download protocol
Standard Protocols
Click here to view our Standard Protocols