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Rat ICAM-1/CD54 Recombinant antibody, PBS Only (Capture)

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240725C2

Cat no : 83696-3-PBS

Print datasheet

Synonyms

CD54, ICAM-1, ICAM-12, Icam1, 240725C2

验证数据展示

  • WB analysis using 83696-3-RR (same clone as 83696-3-PBS)

    WB analysis using 83696-3-RR (same clone as 83696-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

    IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

    IHC staining of rat liver using 83696-3-RR (same clone as 83696-3-PBS)

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 83696-3-RR (ICAM-1/CD54 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83696-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00678-1

    Cytometric bead array standard curve of MP00678-1

    Cytometric bead array standard curve of MP00678-1, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-3-PBS. Detection antibody: 83696-1-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

  • Cytometric bead array standard curve of MP00678-3

    Cytometric bead array standard curve of MP00678-3

    Cytometric bead array standard curve of MP00678-3, RAT ICAM-1/CD54 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83696-3-PBS. Detection antibody: 83696-2-PBS. Standard: Eg1025. Range: 0.156-20 ng/mL.

  • Affinity and Kinetic Characterization of 83696-3-RR

    Affinity and Kinetic Characterization of 83696-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83696-3-RR against Mouse ICAM-1/CD54 were performed. The affinity constant is 1.09 nM.

产品信息

83696-3-PBS targets ICAM-1/CD54 as part of a matched antibody pair:

MP00678-1: 83696-3-PBS capture and 83696-1-PBS detection (validated in Cytometric bead array)

MP00678-3: 83696-3-PBS capture and 83696-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM-1, ICAM-12, Icam1, 240725C2
Calculated Molecular Weight60 kDa
Observed Molecular Weight85 kDa
GenBank Accession NumberNM_012967.1
Gene Symbol Icam1
Gene ID (NCBI) 25464
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ00238
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).