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Anti-Human ICOS/CD278 Rabbit Recombinant Antibody

ICOS/CD278 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241770G11

Cat no : 98214-1-RR

Print datasheet

Synonyms

ICOS, CVID1, CD278, AILIM, Activation-inducible lymphocyte immunomediatory molecule

验证数据展示

  • FC experiment of human PBMCs using 98214-1-RR

    FC experiment of human PBMCs using 98214-1-RR

    1x10^6 untreated or PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ICOS/CD278 Rabbit Recombinant Antibody (98214-1-RR, Clone: 241770G11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

  • FC experiment of human PBMCs using 98214-1-RR

    FC experiment of human PBMCs using 98214-1-RR

    1x10^6 PHA treated human PBMCs were surface stained with 0.25 ug Anti-Human ICOS/CD278 Rabbit Recombinant Antibody (98214-1-RR, Clone: 241770G11) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were surface stained with 0.25 ug Anti-Human ICOS/CD278 Rabbit Recombinant Antibody (98214-1-RR, Clone: 241770G11) (black, dashed). Cells were not fixed.

经过测试的应用

Positive FC detected inPHA treated human PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98214-1-RR targets ICOS/CD278 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name inducible T-cell co-stimulator
Synonyms ICOS, CVID1, CD278, AILIM, Activation-inducible lymphocyte immunomediatory molecule
Calculated Molecular Weight 199 aa, 23 kDa
GenBank Accession NumberBC028210
Gene Symbol ICOS
Gene ID (NCBI) 29851
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y6W8
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13.

实验方案

Product Specific Protocols
FC protocol for ICOS/CD278 antibody 98214-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols