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验证数据展示

  • WB analysis using 67003-1-Ig

    WB analysis using 67003-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67003-1-Ig (ICOSLG antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ICOSLG/B7-H2/CD275 Monoclonal antibody

ICOSLG/B7-H2/CD275 Monoclonal Antibody for WB, ELISA
Cat No. 67003-1-Ig

Print datasheet

CloneNo. 2G1F8

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, ELISA

Synonyms

B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, Daudi cells, HUVEC cells, JAR cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67003-1-Ig targets ICOSLG/B7-H2/CD275 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human
Immunogen ICOSLG/B7-H2/CD275 fusion protein Ag6799 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name inducible T-cell co-stimulator ligand
Synonyms B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50
Calculated Molecular Weight 33 kDa, 34 kDa
Observed Molecular Weight 60-65 kDa, 28-35 kDa
GenBank Accession NumberBC064637
Gene Symbol ICOSLG
Gene ID (NCBI) 23308
RRIDAB_2882320
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75144
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

实验方案

Product Specific Protocols
WB protocol for ICOSLG/B7-H2/CD275 antibody 67003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols