ICOSLG/B7-H2/CD275 Monoclonal antibody

ICOSLG/B7-H2/CD275 Monoclonal Antibody for WB, ELISA
Cat No. 67003-1-Ig

产品说明书

CloneNo. 2G1F8

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

WB, ELISA

B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHL-60 cells, Daudi cells, HUVEC cells, JAR cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67003-1-Ig targets ICOSLG/B7-H2/CD275 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 ICOSLG/B7-H2/CD275 fusion protein Ag6799 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 inducible T-cell co-stimulator ligand
别名 B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50
计算分子量 33 kDa, 34 kDa
观测分子量 60-65 kDa, 28-35 kDa
GenBank蛋白编号BC064637
基因名称 ICOSLG
Gene ID (NCBI) 23308
RRIDAB_2882320
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75144
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

实验方案

Product Specific Protocols
WB protocol for ICOSLG/B7-H2/CD275 antibody 67003-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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