验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, Daudi cells, HUVEC cells, JAR cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
67003-1-Ig targets ICOSLG/B7-H2/CD275 in WB, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human |
免疫原 | ICOSLG/B7-H2/CD275 fusion protein Ag6799 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | inducible T-cell co-stimulator ligand |
别名 | B7H2, ICOSLG, B7RP1, B7-related protein 1, B7-like protein Gl50 |
计算分子量 | 33 kDa, 34 kDa |
观测分子量 | 60-65 kDa, 28-35 kDa |
GenBank蛋白编号 | BC064637 |
基因名称 | ICOSLG |
Gene ID (NCBI) | 23308 |
RRID | AB_2882320 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | O75144 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).
实验方案
Product Specific Protocols | |
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WB protocol for ICOSLG/B7-H2/CD275 antibody 67003-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |