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- KD/KO Validated
IDE Monoclonal antibody
IDE Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat and More (2)
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
3E10G1
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A375 cells, HepG2 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells, A431 cells, HEK-293 cells, MCF-7 cells, NCCIT cells, HT-1080 cells, LNCaP cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
产品信息
67106-1-Ig targets IDE in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | Human, Mouse, Rat |
| Cited Reactivity | human, mouse |
| Immunogen | IDE fusion protein Ag17161 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | Abeta degrading protease |
| Synonyms | Abeta degrading protease, FLJ35968, IDE, INSULYSIN |
| Calculated Molecular Weight | 1019 aa, 118 kDa |
| Observed Molecular Weight | 100-120 kDa |
| GenBank Accession Number | BC096336 |
| Gene Symbol | IDE |
| Gene ID (NCBI) | 3416 |
| RRID | AB_2882410 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P14735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IDE antibody 67106-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging Cell Microglia-derived Galectin-9 drives amyloid-β pathology in Alzheimer's disease | ||
Mol Nutr Food Res Long-Term High-Fat Diet Decreases Renal Insulin-Degrading Enzyme Expression and Function by Inhibiting the PPARγ Pathway | ||
Mol Med Inhibition of insulin degrading enzyme suppresses osteoclast hyperactivity via enhancing Nrf2-dependent antioxidant response in glucocorticoid-induced osteonecrosis of the femoral head | ||
Phytomedicine Osmundacetone ameliorates Alzheimer's-like pathologies by inhibiting β-amyloid fibrillation, oxidative damage and neuroinflammation in APP/PS1 transgenic mice | ||
Phytomedicine Jiawei Xionggui Decoction promotes meningeal lymphatic vessels clearance of β-amyloid by inhibiting arachidonic acid pathway |
