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验证数据展示

  • WB analysis using 66918-1-Ig (same clone as 66918-1-PBS)

    WB analysis using 66918-1-Ig (same clone as 66918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66918-1-Ig (IDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66918-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66918-1-Ig (same clone as 66918-1-PBS)

    WB analysis of HEK-293 using 66918-1-Ig (same clone as 66918-1-PBS)

    WB result of IDH2 antibody (66918-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66918-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66918-1-Ig (same clone as 66918-1-PBS)

    FC experiment of HepG2 using 66918-1-Ig (same clone as 66918-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH2 Monoclonal antibody (66918-1-Ig, Clone:1F10A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Intracellular Staining Permeabilization Wash Buffer. This data was developed using the same antibody clone with 66918-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IDH2 Monoclonal antibody, PBS Only

IDH2 Monoclonal Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 66918-1-PBS

产品说明书

CloneNo. 1F10A10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.

产品信息

66918-1-PBS targets IDH2 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 IDH2 fusion protein Ag8950 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 2 (NADP+), mitochondrial
别名 IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42
计算分子量 452 aa, 51 kDa
观测分子量 41-47 kDa
GenBank蛋白编号BC009244
基因名称 IDH2
Gene ID (NCBI) 3418
RRIDAB_2882245
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48735
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.