验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, K-562 cells |
Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
66918-1-Ig targets IDH2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, FC (Intra), ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | IDH2 fusion protein Ag8950 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
别名 | IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42 |
计算分子量 | 452 aa, 51 kDa |
观测分子量 | 41-47 kDa |
GenBank蛋白编号 | BC009244 |
基因名称 | IDH2 |
Gene ID (NCBI) | 3418 |
RRID | AB_2882245 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P48735 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.
实验方案
Product Specific Protocols | |
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WB protocol for IDH2 antibody 66918-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |