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IDH2 Monoclonal antibody

IDH2 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66918-1-Ig

产品说明书

CloneNo. 1F10A10

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, FC (Intra), ELISA

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HEK-293 cells, HepG2 cells, SH-SY5Y cells, K-562 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66918-1-Ig targets IDH2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 IDH2 fusion protein Ag8950 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 2 (NADP+), mitochondrial
别名 IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42
计算分子量 452 aa, 51 kDa
观测分子量 41-47 kDa
GenBank蛋白编号BC009244
基因名称 IDH2
Gene ID (NCBI) 3418
RRIDAB_2882245
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP48735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

实验方案

Product Specific Protocols
WB protocol for IDH2 antibody 66918-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

RIPK3 deficiency blocks R-2-hydroxyglutarate-induced necroptosis in IDH-mutated AML cells

Authors - Shuanghong Zhu
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