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验证数据展示

  • FC experiment of HepG2 using CL488-66918

    FC experiment of HepG2 using CL488-66918

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated IDH2 Monoclonal antibody (CL488-66918, Clone:1F10A10)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using CL488-66918

    WB analysis using CL488-66918

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66918 (IDH2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IDH2 Monoclonal antibody

IDH2 Monoclonal Antibody for WB, FC (Intra)
Cat No. CL488-66918

Print datasheet

CloneNo. 1F10A10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra)

Synonyms

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66918-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66918 targets IDH2 in WB, FC (Intra) applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra) Application Description
Tested Reactivity human
Immunogen IDH2 fusion protein Ag8950 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight 41-47 kDa
GenBank Accession NumberBC009244
Gene Symbol IDH2
Gene ID (NCBI) 3418
RRIDAB_3084287
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP48735
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 IDH2 antibody CL488-66918Download protocol
Standard Protocols
Click here to view our Standard Protocols