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IDH2 Recombinant antibody, PBS Only

IDH2 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IP, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B1

Cat no : 82117-1-PBS

Print datasheet

Synonyms

ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase

验证数据展示

  • WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

    WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

  • WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

    WB analysis using 82117-1-RR (same clone as 82117-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 82117-1-RR (same clone as 82117-1-PBS)

    IP experiment of mouse brain using 82117-1-RR (same clone as 82117-1-PBS)

    IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 82117-1-RR (same clone as 82117-1-PBS)

    IHC staining of human colon cancer using 82117-1-RR (same clone as 82117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 82117-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH2 antibody included
Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.

产品信息

82117-1-PBS targets IDH2 in WB, IP, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IP, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen IDH2 fusion protein Ag8779 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name isocitrate dehydrogenase 2 (NADP+), mitochondrial
Synonyms ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase
Calculated Molecular Weight 452 aa, 51 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberBC009244
Gene Symbol IDH2
Gene ID (NCBI) 3418
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48735
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.