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验证数据展示

  • WB analysis using 82117-1-RR

    WB analysis using 82117-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82117-1-RR

    WB analysis using 82117-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82117-1-RR (IDH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 82117-1-RR

    WB analysis of HEK-293 using 82117-1-RR

    WB result of IDH2 antibody (82117-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-IDH2 transfected HEK-293 cells.

  • IP experiment of mouse brain using 82117-1-RR

    IP experiment of mouse brain using 82117-1-RR

    IP result of anti-IDH2 (IP:82117-1-RR, 4ug; Detection:82117-1-RR 1:1000) with mouse brain tissue lysate 1280 ug.

  • IHC staining of human colon cancer using 82117-1-RR

    IHC staining of human colon cancer using 82117-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 82117-1-RR (IDH2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

    Ready-to-use IHC kits available with IDH2 antibody included
  • Featured Product
  • KD/KO验证

IDH2 Recombinant antibody

IDH2 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Cat No. 82117-1-RR

产品说明书

CloneNo. 1B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, mouse heart tissue, Jurkat cells, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82117-1-RR targets IDH2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IDH2 fusion protein Ag8779 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 isocitrate dehydrogenase 2 (NADP+), mitochondrial
别名 IDHM, IDH 2, ICD-M, ICD M, EC:1.1.1.42
计算分子量 452 aa, 51 kDa
观测分子量43 kDa
GenBank蛋白编号BC009244
基因名称 IDH2
Gene ID (NCBI) 3418
RRIDAB_3086464
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48735
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.

实验方案

Product Specific Protocols
WB protocol for IDH2 antibody 82117-1-RRDownload protocol
IHC protocol for IDH2 antibody 82117-1-RRDownload protocol
IP protocol for IDH2 antibody 82117-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols