IDH2 Recombinant antibody
IDH2 Uni-rAbTM Recombinant Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
1B1
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, mouse heart tissue, Jurkat cells, rat heart tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82117-1-RR targets IDH2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | IDH2 fusion protein Ag8779 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | isocitrate dehydrogenase 2 (NADP+), mitochondrial |
| Synonyms | ICD M, IDH, IDH2, IDHM, IDP, IDPM, mNADP IDH, NADP(+) specific ICDH, Oxalosuccinate decarboxylase |
| Calculated Molecular Weight | 452 aa, 51 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC009244 |
| Gene Symbol | IDH2 |
| Gene ID (NCBI) | 3418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P48735 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IDH2 antibody 82117-1-RR | Download protocol |
| IHC protocol for IDH2 antibody 82117-1-RR | Download protocol |
| IP protocol for IDH2 antibody 82117-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |