IDH3B Monoclonal antibody

IDH3B Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Rabbit, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2D11A9

Cat no : 68199-1-Ig

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Synonyms

FLJ11043, H IDHB, IDH3B, RP46



经过测试的应用

Positive WB detected inLNCaP cells, Jurkat cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, pig heart tissue, rabbit heart tissue, rat heart tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68199-1-Ig targets IDH3B in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Rabbit, Pig samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Rabbit, Pig
Immunogen IDH3B fusion protein Ag8693 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name isocitrate dehydrogenase 3 (NAD+) beta
Synonyms FLJ11043, H IDHB, IDH3B, RP46
Calculated Molecular Weight 42 kDa
Observed Molecular Weight39-42 kDa
GenBank Accession NumberBC001960
Gene Symbol IDH3B
Gene ID (NCBI) 3420
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO43837
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Isocitrate dehydrogenase 3 (IDH3) is a key enzyme in the mitochondrial tricarboxylic acid (TCA) cycle, which catalyzes the decarboxylation of isocitrate into α-ketoglutarate and concurrently converts NAD+ into NADH. IDH3B(Isocitrate dehydrogenase 3 (NAD+) beta), also named as RP46, is the beta subunit of IDH3 and controls its substrate levels in the TCA cycle, and it is required for sperm mitochondrial metabolism and spermiogenesis (PMID: 18806796, PMID: 35985423). In addition, DH3B expression increased PFKFB3 protein levels and enhanced glucose uptake, and high expression of IDH3B correlated with poor survival in patients with ESCC, suggesting a potential application of IDH3B in prognosis (PMID: 31053633).

实验方案

Product Specific Protocols
WB protocol for IDH3B antibody 68199-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols