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CoraLite® Plus 488-conjugated IDO1 Monoclonal antibody

IDO1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3G2G11

Cat no : CL488-66528

Print datasheet

Synonyms

CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1

验证数据展示

  • WB analysis using CL488-66528

    WB analysis using CL488-66528

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66528 (IDO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inIFN gamma treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy IDO1 Ready-To-Use IHC Kit. IDO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66528-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66528 targets IDO1 in WB applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity Human, mouse, rat
Immunogen IDO1 fusion protein Ag3953 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name indoleamine 2,3-dioxygenase 1
Synonyms CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP14902
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 IDO1 antibody CL488-66528Download protocol
Standard Protocols
Click here to view our Standard Protocols