验证数据展示
经过测试的应用
| Positive WB detected in | IFN gamma treated HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy IDO1 Ready-To-Use IHC Kit. IDO1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66528-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66528 targets IDO1 in WB applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human, mouse, rat |
| 免疫原 | IDO1 fusion protein Ag3953 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | indoleamine 2,3-dioxygenase 1 |
| 别名 | CD107B, IDO, IDO 1, IDO1, INDO, indoleamine 2,3 dioxygenase 1 |
| 计算分子量 | 403 aa, 45 kDa |
| 观测分子量 | 42 kDa |
| GenBank蛋白编号 | BC027882 |
| 基因名称 | IDO1 |
| Gene ID (NCBI) | 3620 |
| RRID | AB_2934476 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P14902 |
| 储存缓冲液 | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 IDO1 antibody CL488-66528 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |