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IFI16 Monoclonal antibody, PBS Only

IFI16 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C10D7

Cat no : 67790-1-PBS

Print datasheet

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2

验证数据展示

  • WB analysis using 67790-1-Ig (same clone as 67790-1-PBS)

    WB analysis using 67790-1-Ig (same clone as 67790-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67790-1-Ig (IFI16 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFI16 antibody included
  • IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    IHC staining of human tonsillitis using 67790-1-Ig (same clone as 67790-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67790-1-Ig (IFI16 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IFI16 antibody included
  • IF Staining of HeLa using 67790-1-Ig (same clone as 67790-1-PBS)

    IF Staining of HeLa using 67790-1-Ig (same clone as 67790-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI16 antibody (67790-1-Ig, Clone: 1C10D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67790-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.

产品信息

67790-1-PBS targets IFI16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen IFI16 fusion protein Ag30314 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon, gamma-inducible protein 16
Synonyms Ifi 16, IFI16, IFNGIP1, PYHIN2
Calculated Molecular Weight 729 aa, 82 kDa
Observed Molecular Weight85-95 kDa
GenBank Accession NumberBC017059
Gene Symbol IFI16
Gene ID (NCBI) 3428
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16666
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)