验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21775-1-AP
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-21775 targets IFIH1 in IF-P applications and shows reactivity with human, mouse samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | IFIH1 fusion protein Ag16467 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | interferon induced with helicase C domain 1 |
| Synonyms | MDA5, Interferon-induced helicase C domain-containing protein 1, IFIH 1, Helicard, EC:3.6.4.13 |
| Calculated Molecular Weight | 1025 aa, 117 kDa |
| Observed Molecular Weight | 117-125 kDa |
| GenBank Accession Number | BC111750 |
| Gene Symbol | IFIH1 |
| Gene ID (NCBI) | 64135 |
| RRID | AB_2883087 |
| Conjugate | CoraLite®488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 491 nm / 516 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYX4 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL488 IFIH1 antibody CL488-21775 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
