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验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-21775 (IFIH1 antibody) at dilution of 1:100.

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®488-conjugated IFIH1 Polyclonal antibody

IFIH1 Polyclonal Antibody for IF-P
Cat No. CL488-21775

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF-P

别名

MDA5, Interferon-induced helicase C domain-containing protein 1, IFIH 1, Helicard, EC:3.6.4.13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21775-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21775 targets IFIH1 in IF-P applications and shows reactivity with human, mouse samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse
免疫原 IFIH1 fusion protein Ag16467 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon induced with helicase C domain 1
别名 MDA5, Interferon-induced helicase C domain-containing protein 1, IFIH 1, Helicard, EC:3.6.4.13
计算分子量 1025 aa, 117 kDa
观测分子量117-125 kDa
GenBank蛋白编号BC111750
基因名称 IFIH1
Gene ID (NCBI) 64135
RRIDAB_2883087
偶联类型 CoraLite®488 Fluorescent Dye
最大激发/发射波长491 nm / 516 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYX4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

实验方案

Product Specific Protocols
IF protocol for CL488 IFIH1 antibody CL488-21775Download protocol
Standard Protocols
Click here to view our Standard Protocols