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  • Featured Product
  • KD/KO Validated

CoraLite®488-conjugated IFIH1 Polyclonal antibody

IFIH1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF-P

Conjugate

CoraLite®488 Fluorescent Dye

Cat no : CL488-21775

Print datasheet

Synonyms

MDA5, Interferon-induced helicase C domain-containing protein 1, IFIH 1, Helicard, EC:3.6.4.13

验证数据展示

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CL488-21775 (IFIH1 antibody) at dilution of 1:100.

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

  • IF Staining of mouse skeletal muscle using CL488-21775

    IF Staining of mouse skeletal muscle using CL488-21775

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using CoraLite®488 IFIH1 antibody (CL488-21775) at dilution of 1:200.

经过测试的应用

Positive IF-P detected inmouse skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21775-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-21775 targets IFIH1 in IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse
Immunogen IFIH1 fusion protein Ag16467 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon induced with helicase C domain 1
Synonyms MDA5, Interferon-induced helicase C domain-containing protein 1, IFIH 1, Helicard, EC:3.6.4.13
Calculated Molecular Weight 1025 aa, 117 kDa
Observed Molecular Weight117-125 kDa
GenBank Accession NumberBC111750
Gene Symbol IFIH1
Gene ID (NCBI) 64135
RRIDAB_2883087
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYX4
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.

实验方案

Product Specific Protocols
IF protocol for CL488 IFIH1 antibody CL488-21775Download protocol
Standard Protocols
Click here to view our Standard Protocols