验证数据展示
经过测试的应用
| Positive WB detected in | TF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60074-1-Ig targets IFITM1-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse |
| 免疫原 | IFITM1-Specific fusion protein Ag2320 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | interferon induced transmembrane protein 1 (9-27) |
| 别名 | 9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13 |
| 计算分子量 | 14 kDa |
| 观测分子量 | 14-17 kDa |
| GenBank蛋白编号 | BC000897 |
| 基因名称 | IFITM1 |
| Gene ID (NCBI) | 8519 |
| RRID | AB_2233405 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P13164 |
| 储存缓冲液 | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IHC protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IF protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IP protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| FC protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise. | ||
Cell The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. | ||
Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells | ||
Sci Transl Med Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition. | ||
Cell Host Microbe Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
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