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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated IFITM1-Specific Monoclonal antibody

IFITM1-Specific Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5B5E2

Cat no : CL488-60074

Print datasheet

Synonyms

9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13

验证数据展示

  • IF Staining of K-562 using CL488-60074

    IF Staining of K-562 using CL488-60074

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 IFITM1-Specific antibody (CL488-60074, Clone: 5B5E2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy IFITM1 Ready-To-Use IHC Kit. IFITM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60074-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60074 targets IFITM1-Specific in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen IFITM1-Specific fusion protein Ag2320 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interferon induced transmembrane protein 1 (9-27)
Synonyms 9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa, 17 kDa
GenBank Accession NumberBC000897
Gene Symbol IFITM1
Gene ID (NCBI) 8519
RRIDAB_2919229
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IFITM1-Specific antibody CL488-60074Download protocol
Standard Protocols
Click here to view our Standard Protocols