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验证数据展示

  • FC experiment of human PBMCs using 98187-1-RR

    FC experiment of human PBMCs using 98187-1-RR

    1x10^6 untreated or PMA and ionomycin treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IFN-gamma Rabbit Recombinant Antibody (98187-1-RR, Clone: 241390B10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of human PBMCs using 98187-1-RR

    FC experiment of human PBMCs using 98187-1-RR

    1x10^6 PMA and ionomycin treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IFN-gamma Rabbit Recombinant Antibody (98187-1-RR, Clone: 241390B10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). 1x10^6 untreated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IFN-gamma Rabbit Recombinant Antibody (98187-1-RR, Clone: 241390B10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (black, dashed). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98187-1-RR

    Affinity and Kinetic Characterization of 98187-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98187-1-RR against Human IFN-gamma were performed. The affinity constant is below 1 pM.

Anti-Human IFN-gamma Rabbit Recombinant Antibody

IFN-gamma Recombinant Antibody for FC (Intra)
Cat No. 98187-1-RR

产品说明书

CloneNo. 241390B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra)

别名

IFNG, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma, IFG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPMA and ionomycin treated human PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98187-1-RR targets IFN-gamma in FC (Intra) applications and shows reactivity with human samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IFN gamma
别名 IFNG, IFN-gamma,IFN gamma,IFN γ,IFNγ, IFN γ, IFN gamma, IFG
计算分子量 166 aa, 19 kDa
GenBank蛋白编号BC070256
基因名称 IFNG
Gene ID (NCBI) 3458
ENSEMBL Gene IDENSG00000111537
RRIDAB_3672327
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP01579
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins. (PMID: 19268625; 10688427)

实验方案

Product Specific Protocols
FC protocol for IFN-gamma antibody 98187-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols