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IFN-gamma R1 Monoclonal antibody

IFN-gamma R1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1H3A4

Cat no : 66704-1-Ig

Print datasheet

Synonyms

IFNGR1, IFN-gamma-R1, IFN-gamma receptor 1, IFN gamma receptor 1, IFN gamma R1

验证数据展示

  • WB analysis of HeLa using 66704-1-Ig

    WB analysis of HeLa using 66704-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, T-47D cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66704-1-Ig targets IFN-gamma R1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen IFN-gamma R1 fusion protein Ag27085 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interferon gamma receptor 1
Synonyms IFNGR1, IFN-gamma-R1, IFN-gamma receptor 1, IFN gamma receptor 1, IFN gamma R1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC005333
Gene Symbol IFNGR1
Gene ID (NCBI) 3459
RRIDAB_2882056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

实验方案

Product Specific Protocols
WB protocol for IFN-gamma R1 antibody 66704-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols