验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, mouse testis tissue, rat testis tissue |
| Positive IP detected in | rat testis tissue |
| Positive IHC detected in | mouse testis tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse testis tissue |
| Positive IF/ICC detected in | C2C12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17460-1-AP targets IFT140 in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, canine, zebrafish |
| 免疫原 | IFT140 fusion protein Ag11119 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | intraflagellar transport 140 homolog (Chlamydomonas) |
| 别名 | WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog |
| 计算分子量 | 1462 aa, 165 kDa |
| 观测分子量 | 150-165 kDa |
| GenBank蛋白编号 | BC035577 |
| 基因名称 | IFT140 |
| Gene ID (NCBI) | 9742 |
| RRID | AB_2295648 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96RY7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFT140 antibody 17460-1-AP | Download protocol |
| IHC protocol for IFT140 antibody 17460-1-AP | Download protocol |
| IF protocol for IFT140 antibody 17460-1-AP | Download protocol |
| IP protocol for IFT140 antibody 17460-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Genet Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy. | ||
Cell Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision. | ||
Sci Adv Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ. | ||
Nat Commun A distal centriolar protein network controls organelle maturation and asymmetry. | ||
Nat Commun Kinesin-2 and IFT-A act as a complex promoting nuclear localization of β-catenin during Wnt signalling. | ||
Nat Commun IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning. |
