• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated IFT140 Polyclonal antibody

IFT140 Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-17460

Print datasheet

Synonyms

WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog

验证数据展示

  • IF Staining of mouse testis using CL594-17460

    IF Staining of mouse testis using CL594-17460

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

  • IF Staining of mouse testis using CL594-17460

    IF Staining of mouse testis using CL594-17460

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200.

  • IF Staining of hTERT-RPE1 using CL594-17460

    IF Staining of hTERT-RPE1 using CL594-17460

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CoraLite®594 IFT140 antibody (CL594-17460) at dilution of 1:200, CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inhTERT-RPE1 cells
For other applications, we recommend the unconjugated version of this antibody, 17460-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17460 targets IFT140 in IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen IFT140 fusion protein Ag11119 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 140 homolog (Chlamydomonas)
Synonyms WDTC2, KIAA0590, Intraflagellar transport protein 140 homolog
Calculated Molecular Weight 1462 aa, 165 kDa
GenBank Accession NumberBC035577
Gene Symbol IFT140
Gene ID (NCBI) 9742
RRIDAB_2919840
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96RY7
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFT140 is a subunit of intraflagellar transport complex A (IFT-A) which is involved in retrograde ciliary transport. RT-PCR analysis showed it is highly expressed in kidney, moderately in ovary, testis, prostate, and lung. IFT140 is localised to the base and tip of primary cilium. IFT140 has a pivotal role in development and function of ciliated cells, and mutations of IFT140 cause skeletal, renal, and retinal ciliopathies. It had been detected as a single band around 140-165 kDa in different reports. (PMID: 20368623, 22282595)

实验方案

Product Specific Protocols
IF protocol for CL594 IFT140 antibody CL594-17460Download protocol
Standard Protocols
Click here to view our Standard Protocols