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验证数据展示

  • WB analysis of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

    WB analysis of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • WB analysis using 11744-1-AP (same clone as 11744-1-PBS)

    WB analysis using 11744-1-AP (same clone as 11744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

    IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

    IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11744-1-AP (same clone as 11744-1-PBS)

    IHC staining of human prostate cancer using 11744-1-AP (same clone as 11744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of C2C12 using 11744-1-AP (same clone as 11744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of hTERT-RPE1 using 11744-1-AP (same clone as 11744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

IFT81 Polyclonal antibody, PBS Only

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11744-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

11744-1-PBS targets IFT81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原 IFT81 fusion protein Ag2339 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 intraflagellar transport 81 homolog (Chlamydomonas)
别名
计算分子量 676 aa, 80 kDa
观测分子量 75-80 kDa
GenBank蛋白编号BC029349
基因名称 IFT81
Gene ID (NCBI) 28981
RRIDAB_2121966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYA0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.