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  • Featured Product
  • KD/KO Validated

IFT81 Polyclonal antibody, PBS Only

IFT81 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 11744-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

    WB analysis of mouse testis using 11744-1-AP (same clone as 11744-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • WB analysis using 11744-1-AP (same clone as 11744-1-PBS)

    WB analysis using 11744-1-AP (same clone as 11744-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11744-1-AP (IFT81 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF result (localization to the spindle poles) of anti-IFT81 (11744-1-AP, 1:50) with metaphase hTERT-RPE1 cells (MeOH fixed) byDr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of hTERT-RPE1 cells using 11744-1-AP (same clone as 11744-1-PBS)

    IF result (the base and tip of cilia) of anti-IFT81 (11744-1-AP, 1:50) with serum-starved hTERT-RPE1 (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

    IP experiment of mouse brain using 11744-1-AP (same clone as 11744-1-PBS)

    IP result of anti-IFT81 (IP:11744-1-AP, 3ug; Detection:11744-1-AP 1:500) with mouse brain tissue lysate 7500ug. This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 11744-1-AP (same clone as 11744-1-PBS)

    IHC staining of human prostate cancer using 11744-1-AP (same clone as 11744-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11744-1-AP (IFT81 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of MDCK using 11744-1-AP (same clone as 11744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of C2C12 using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of C2C12 using 11744-1-AP (same clone as 11744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT81 antibody (11744-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 acetylated Tubulin(Lys40) antibody (CL594-66200, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 11744-1-AP (same clone as 11744-1-PBS)

    IF Staining of hTERT-RPE1 using 11744-1-AP (same clone as 11744-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT81 antibody (11744-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red). This data was developed using the same antibody clone with 11744-1-PBS in a different storage buffer formulation.

产品信息

11744-1-PBS targets IFT81 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, canine
Immunogen IFT81 fusion protein Ag2339 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intraflagellar transport 81 homolog (Chlamydomonas)
Synonyms
Calculated Molecular Weight 676 aa, 80 kDa
Observed Molecular Weight 75-80 kDa
GenBank Accession NumberBC029349
Gene Symbol IFT81
Gene ID (NCBI) 28981
RRIDAB_2121966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WYA0
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT particles are multi-subunit complexes of proteins that functions to move non-membrane-bound particles from the cell body to the tip of cilium or flagellum, then return them to the cell body. Transport towards the ciliary tip is regulated by the IFT complex B (IFT-B), consisting of at least 15 IFT proteins, in association with kinesin motors, whereas transport from the ciliary tip back to the base is executed by a dynein motor in association with the IFT complex A (IFT-A), currently known to be composed of six IFT proteins. IFT81 is a subunit of IFT complex B.It may play a role in development of the testis and spermatogenesis. There are some isoforms of IFT81 with 73-78 kDa and 43-50 kDa.