IGF2BP3 Recombinant antibody
IGF2BP3 Uni-rAbTM Recombinant Antibody for WB, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
1L11
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells, mouse placenta tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human pancreas cancer tissue, human cervical squamous cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
81805-1-RR targets IGF2BP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IP, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Immunogen | IGF2BP3 fusion protein Ag6226 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | insulin-like growth factor 2 mRNA binding protein 3 |
Synonyms | IMP3, IMP 3, IGF-II mRNA-binding protein 3, IGF2BP 3, IGF2 mRNA-binding protein 3 |
Calculated Molecular Weight | 64 kDa |
Observed Molecular Weight | 64 kDa |
GenBank Accession Number | BC065269 |
Gene Symbol | IGF2BP3 |
Gene ID (NCBI) | 10643 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | O00425 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IGF2BP3 antibody 81805-1-RR | Download protocol |
IHC protocol for IGF2BP3 antibody 81805-1-RR | Download protocol |
IP protocol for IGF2BP3 antibody 81805-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |